Faculty Advisor

Adams, David S.

Abstract

Aerobic respiration produces reactive oxygen species which can cause DNA lesions, which lead to mutation. This project's goal is to detect DNA damage due to the presence/absence of the protein Oxr1, which regulates ROS levels in cells. This is done by growing yeast strains that contain wild type and mutant Oxr1 and Ogg1 alleles. Ogg1 is a repair protein which repairs ROS caused lesions. These cultures are plated on arginine deficient minimal media and the same media with canavanine to measure mutation frequency. Colonies are counted and mutation frequency is calculated for each strain to determine which genotypes have higher mutation frequencies and are thus less efficient at reducing spontaneous mutagenesis. Results indicated that Oxr1 plays a small role in spontaneous mutagenesis.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2008

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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