Numerical and experimental investigations of electrophoresis of bovine hemoglobin in a two-phase system were conducted. The numerical model was generated in COMSOL 3.5 as a 2-D representation of a U-tube, assuming equilibrium at the phase interface, using electro-kinetic flow and conductive media DC equations. The experimental separation of the protein was conducted in a PEG-dextran two-phase solution in a U-tube, using an induced current up to 3kV. Both methods resulted in a successful migration of the protein across the interphase.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Mercer, Brian Cameron
• Laflash, Andrew Jordon
• Newman, Erik Joseph
• Maziarz, Anna M.

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042811-121650

Advisor

• Clark, William M.

Year

• 2011

Date created

• 2011-04-28

Resource type

• Major Qualifying Project

Major

• Chemical Engineering

Rights statement

• In Copyright