Faculty Advisor

Clark, William M.

Abstract

Numerical and experimental investigations of electrophoresis of bovine hemoglobin in a two-phase system were conducted. The numerical model was generated in COMSOL 3.5 as a 2-D representation of a U-tube, assuming equilibrium at the phase interface, using electro-kinetic flow and conductive media DC equations. The experimental separation of the protein was conducted in a PEG-dextran two-phase solution in a U-tube, using an induced current up to 3kV. Both methods resulted in a successful migration of the protein across the interphase.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2011

Major

Chemical Engineering

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Chemical Engineering

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