Faculty Advisor

Adams, David S.

Center

Worcester, MA

Abstract

Oligosaccharyl Transferase (OST) N-glycosylates nascent polypeptides as they are being translocated into the endoplasmic reticulum (ER). Mammalian OST contains eight subunits, of which STT3 is believed to be the catalytic one. There are two isoforms of STT3, STT3-A and STT3-B, which have different kinetic and substrate recognition properties. Interestingly, it was found that both isoforms are present in cells from a variety of tissues. However, it is unclear if both are needed for proper functioning of OST. RNAi will be used to knockdown expression of one of the isoforms in HEK293 cells to measure differing effects in N-glycosylation of proteins.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2005

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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