Faculty Advisor

Duffy, Joseph B.

Abstract

One of the main functions of a tumor suppressor is to repress cell division, sometimes by inducing cellular senescence. Smurf2 and Notch3 have previously been identified in our laboratory as tumor suppressors in vitro. It is not well understood how these genes regulate senescence. The purpose of this project is to better understand the mechanisms underlying the functions of these genes in senescence. A genetic screen using short hairpin RNAs (shRNAs) was carried out to identify genes downstream of Smurf2 or Notch3 in the senescence pathways. Five candidate genes were further analyzed in fibroblasts. To characterize the function of Smurf2 in senescence in vivo, a Cre-LoxP system was used to study the consequence of Smurf2 overexpression in mice.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2012

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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