Faculty Advisor

Weathers, Pamela

Abstract

Our project analyzed root and shoot elicitation of the anti-malarial drug-producing plant, Artemisia annua, using the elicitors chitosan and salicylic acid. We accomplished this by applying chitosan and salicylic acid to the roots and shoots of hydroponically grown A. annua and harvesting both the tips of the apical meristem and the third through fifth leaves from the tips. The antimalarial drug, artemisinin, and flavonoids that act synergistically with the drug were extracted and quantified using GC/MS and an aluminum chloride assay, respectively. We observed higher concentrations of artemisinin in the tips of plants treated with chitosan via the roots indicating chitosan elicits artemisinin biosynthesis and that the roots play an important role in the production of artemisinin.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2013

Major

Biology and Biotechnology

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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