Faculty Advisor

Adams, David S.

Abstract

Over-expression of Vascular Endothelial Growth Factor-A (VEGF-A) can lead to a variety of human diseases, including different forms of cancer, psoriasis, and arthritis. The goal of this project was to develop a cell culture-based assay using human umbilical vein endothelial cells (HUVECs) to identify potential inhibitors of the VEGF-Receptor-2 (VEGFR2) and potential blockers of angiogenesis. After testing a variety of assays, the Hoechst nuclear stain was found to be convenient assays for quantifying cell proliferation. A screen of a chemical compound library using this Hoechst assay identified BSB660 as a novel inhibitor of VEGFR2. In the future, other chemical libraries will be tested using a TDA in vitro assay under development by other Blue Sky employees.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2013

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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