P-type ATPases are a family of enzymes responsible for the transport of lipids and ions across membranes. Although models exist for these enzymes, knowledge on specific transporters is more often than not incomplete. This study sought to improve the knowledge of certain Mycobacterium tuberculosis ATPases through biochemical characterizations using ATPase activity assays and in vivo effects of the proteins on bacterial metal resistance. In addition, protocols for detergent solubilization, micellar reconstitution, and isolation were optimized. Purified CtpG showed evidence of activity in the presence of copper; however, none of the other proteins' biochemical studies or in vivo explorations yielded clear results on the effect of these proteins on the metal resistance of the bacterium.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Kim, Hyungjoon

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042709-104847

Advisor

• Argüello, José M.

Year

• 2009

Date created

• 2009-04-27

Resource type

• Major Qualifying Project

Major

• Biochemistry

Rights statement

• In Copyright

Last modified

• 2021-01-27