Arguello, Jose' M
P-type ATPases are a family of enzymes responsible for the transport of lipids and ions across membranes. Although models exist for these enzymes, knowledge on specific transporters is more often than not incomplete. This study sought to improve the knowledge of certain Mycobacterium tuberculosis ATPases through biochemical characterizations using ATPase activity assays and in vivo effects of the proteins on bacterial metal resistance. In addition, protocols for detergent solubilization, micellar reconstitution, and isolation were optimized. Purified CtpG showed evidence of activity in the presence of copper; however, none of the other proteins' biochemical studies or in vivo explorations yielded clear results on the effect of these proteins on the metal resistance of the bacterium.
Worcester Polytechnic Institute
Major Qualifying Project
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Chemistry and Biochemistry