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An Investigation of the Porcine Circovirus Type 1 Third Viral Protein Nuclear Export Capability Through Fluorescence Microscopy and Rapid Prototyping

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PCV1 VP3 has anti-cancer properties similar to Apoptin, a well known viral protein that induces apoptosis in cancerous cell. Apoptin is known to be CRM-1 dependent, while PCV1 VP3 only has circumstantial evidence. To test CRM-1 dependence for PCV1 VP3, a competitive CRM-1 NES binding site inhibitor, leptomycin B (LMB), was added to cells transfected with PCV1 VP3 DNA or DNA containing only the tail region. Three-dimensional printing was also experimented with to test induced fit of CRM-1 to NES sites in lieu of traditional computer modeling simulations. The data shows that LMB had a slight effect on the tail region of PCV1 VP3, which leads us to believe that there may be something else responsible for CRM-1 binding besides the tail NES.

  • This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
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  • E-project-050114-110804
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  • 2014
Date created
  • 2014-05-01
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