Faculty Advisor

Heilman, Destin

Abstract

PCV1 VP3 has anti-cancer properties similar to Apoptin, a well known viral protein that induces apoptosis in cancerous cell. Apoptin is known to be CRM-1 dependent, while PCV1 VP3 only has circumstantial evidence. To test CRM-1 dependence for PCV1 VP3, a competitive CRM-1 NES binding site inhibitor, leptomycin B (LMB), was added to cells transfected with PCV1 VP3 DNA or DNA containing only the tail region. Three-dimensional printing was also experimented with to test induced fit of CRM-1 to NES sites in lieu of traditional computer modeling simulations. The data shows that LMB had a slight effect on the tail region of PCV1 VP3, which leads us to believe that there may be something else responsible for CRM-1 binding besides the tail NES.

Publisher

Worcester Polytechnic Institute

Date Accepted

May 2014

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Chemistry and Biochemistry

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