Faculty Advisor

Heilman, Destin

Abstract

PCV1 VP3, is homologous to Apoptin. Apoptin is known to self-multimerize and induce apoptosis in cancerous cells. Due to their similarities, it is important to determine if multimerization is a crucial component in the apoptosis. To investigate this, GFP and FLAG tagged VP3 constructs of PCV1 were transfected into H1299 cells. The VP3 interaction was then analyzed using co- immunoprecipitation and flow cytometry. Preliminary analysis showed that PCV1 VP3 does not multimerize, however further experiments are required to confirm this.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2015

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Chemistry and Biochemistry

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