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Evaluating Multimerization of Apoptin Homologs

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PCV1 VP3, is homologous to Apoptin. Apoptin is known to self-multimerize and induce apoptosis in cancerous cells. Due to their similarities, it is important to determine if multimerization is a crucial component in the apoptosis. To investigate this, GFP and FLAG tagged VP3 constructs of PCV1 were transfected into H1299 cells. The VP3 interaction was then analyzed using co- immunoprecipitation and flow cytometry. Preliminary analysis showed that PCV1 VP3 does not multimerize, however further experiments are required to confirm this.

  • This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
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  • E-project-043015-114355
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  • 2015
Date created
  • 2015-04-30
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