PCV1 VP3, is homologous to Apoptin. Apoptin is known to self-multimerize and induce apoptosis in cancerous cells. Due to their similarities, it is important to determine if multimerization is a crucial component in the apoptosis. To investigate this, GFP and FLAG tagged VP3 constructs of PCV1 were transfected into H1299 cells. The VP3 interaction was then analyzed using co- immunoprecipitation and flow cytometry. Preliminary analysis showed that PCV1 VP3 does not multimerize, however further experiments are required to confirm this.
Worcester Polytechnic Institute
Major Qualifying Project
All authors have granted to WPI a nonexclusive royalty-free license to distribute copies of the work, subject to other agreements. Copyright is held by the author or authors, with all rights reserved, unless otherwise noted.
Chemistry and Biochemistry
Your accessibility may vary due to other restrictions.