Faculty Advisor

Dominko, Tanja

Faculty Advisor

Kmiotek, Stephen J.

Faculty Advisor

Page, Raymond L.

Center

OTHER / Other location, not listed

Abstract

Peripheral neural degenerative injuries lead to the formation of fibrotic scars at the site of the lesion which limits axonal regeneration resulting in loss of motor and sensory functionality. This project aims to investigate axonal extension through these fibrotic scars. In order to accomplish this, a device of PDMS and nitrocellulose membrane was designed to simulate the site of injury with a fibrotic scar. Fibroblast NIH 3T3 cells were seeded onto the nitrocellulose membrane to simulate the fibrotic scar. This scar was treated Fibroblast Growth Factor 2 (FGF2) in order to create a less dense extracellular matrix composition. To avoid unwanted fibroblast interaction with the neuron culture, the scar was decellularized before implementation into the device.

Publisher

Worcester Polytechnic Institute

Date Accepted

January 2016

Major

Biomedical Engineering

Major

Chemical Engineering

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

Advisor Department

Chemical Engineering

Advisor Department

Biomedical Engineering

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