Faculty Advisor

Heilman, Destin

Faculty Advisor

Wobbe, Kristin K.

Abstract

Pharmaceuticals that target cancer are becoming ineffective due to mutations in their target proteins. However, oncotropic, oncolytic, and oncoapoptotic viruses exist in nature. Apoptin, for example, from the chicken Anemia Virus (CAV), induces apoptosis in transformed cells by nuclear localization. Porcince Circovirus type 1 (PCV1), from the same family as CAV, contains a third open reading (ORF3) that is twice as long as in its homolog Porcine Circovirus type 2 or Apoptin. Localization and apoptosis analysis were performed on the protein coded in PCV1 ORF3, revealing cytoplasmic localization in both primary and transformed cells, contrary to Apoptin, but with similar apoptosis activity. These results suggest an alternative pathway of apoptosis induction from Apoptin.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2012

Major

Interdisciplinary

Major

Chemistry

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Chemistry and Biochemistry

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