Arguello, Jose' M.
PIB-ATPases are transmembrane enzymes which couple the efflux of cytosolic metals to the hydrolysis of ATP. To study the roles of a putative Ni-transporting ATPase (XinT2), we analyzed the Nickel content of the symbiotic bacteria Sinorhizobium meliloti. Our lab has previously shown that the xinT2::mTn5 mutant strain exhibits sensitivity to nickel. In this work we challenged S. meliloti wild type and mutant cells with sub lethal concentrations of Ni and Fe and observed that deletion of XinT2 lead to cytosolic accumulation of both metals.
Worcester Polytechnic Institute
Major Qualifying Project
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Chemistry and Biochemistry