Faculty Advisor
Roberts, Susan Celia
Abstract
Plant cell suspension cultures have applications in the biopharmaceutical industry as potential vehicles for production of recombinant proteins, but application of this technology remains limited. This project developed two plant cell platforms for recombinant protein synthesis: 1) a carrot cell suspension culture from seed and 2) optimal processing conditions for Arabidopsis suspension cultures. The key outcomes of this research are: 1) establishment of a successful, healthy carrot suspension culture, 2) determination that Arabidopsis suspension cultures experience the highest growth rates in larger flasks with no light exposure, and 3) determination that specialized metabolite production in the cultures is low, indicating that the cells were not under conditions that induce stress.
Publisher
Worcester Polytechnic Institute
Date Accepted
April 2017
Major
Chemical Engineering
Project Type
Major Qualifying Project
Copyright Statement
All authors have granted to WPI a nonexclusive royalty-free license to distribute copies of the work, subject to other agreements. Copyright is held by the author or authors, with all rights reserved, unless otherwise noted.
Accessibility
Unrestricted
Advisor Department
Chemical Engineering