Faculty Advisor

Roberts, Susan Celia

Abstract

Plant cell suspension cultures have applications in the biopharmaceutical industry as potential vehicles for production of recombinant proteins, but application of this technology remains limited. This project developed two plant cell platforms for recombinant protein synthesis: 1) a carrot cell suspension culture from seed and 2) optimal processing conditions for Arabidopsis suspension cultures. The key outcomes of this research are: 1) establishment of a successful, healthy carrot suspension culture, 2) determination that Arabidopsis suspension cultures experience the highest growth rates in larger flasks with no light exposure, and 3) determination that specialized metabolite production in the cultures is low, indicating that the cells were not under conditions that induce stress.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2017

Major

Chemical Engineering

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Chemical Engineering

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