Faculty Advisor

Vidali, Luis

Abstract

Polarized growth, or the delivery of cellular components to only the tip of an expanding cell, is an important process in several specialized cell types. It is mediated in part by the molecular motor myosin, which delivers materials to the tip via transport on actin filaments, and Rab proteins, which are small GTPases hypothesized to function as myosin receptors on secretory vesicles. Protonema cells of the moss P. patens are used to study the proposed interaction between myosin and Rabs. Previously, three residues in myosin were identified by complementation testing as potential sites for this interaction; in this study, expression of mutated myosin is confirmed by fluorescence microscopy and the localization of a type of Rab protein is studied by confocal microscopy.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2013

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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