Porcine Circovirus 1 (PCV1), a non-virulent member of the Circoviridae family, has been found to possess similar genome organization to Chicken Anemia Virus (CAV) and PCV2. The VP3 protein of these viruses induces apoptosis strictly in transformed cells. Though there is sequences homology, PCV1 VP3 has an elongated C-terminus tail domain. In order to elucidate the relationship between the structure and function of PCV1 VP3, truncation mutants isolating the tail domain and the NES were developed. Expression of GFP-tagged PCV1 VP3 in primary and transformed H1299 cells will show the functionality and localization patterns of these mutants. Our research indicates that in transformed cells the tail has a cytoplasmic, vesicular localization while the NES is mainly found in the cytoplasm.
Worcester Polytechnic Institute
Major Qualifying Project
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Chemistry and Biochemistry