Porcine Circovirus 1 (PCV1), a non-virulent member of the Circoviridae family, has been found to possess similar genome organization to Chicken Anemia Virus (CAV) and PCV2. The VP3 protein of these viruses induces apoptosis strictly in transformed cells. Though there is sequences homology, PCV1 VP3 has an elongated C-terminus tail domain. In order to elucidate the relationship between the structure and function of PCV1 VP3, truncation mutants isolating the tail domain and the NES were developed. Expression of GFP-tagged PCV1 VP3 in primary and transformed H1299 cells will show the functionality and localization patterns of these mutants. Our research indicates that in transformed cells the tail has a cytoplasmic, vesicular localization while the NES is mainly found in the cytoplasm.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Paris, Meaghan Hughes
• Zelic, Matija

Contributors

• Spada, Lauren

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042711-115440

Advisor

• Heilman, Destin

Year

• 2011

Date created

• 2011-04-27

Resource type

• Major Qualifying Project

Major

• Biochemistry

Rights statement

• In Copyright