Faculty Advisor

Buckholt, Michael Allan

Faculty Advisor

Gibson, Daniel G

Abstract

Previous MQP studies on horseshoe crab muscles provided several lines of evidence that acetylcholine (ACh) is their neuromuscular junction (NMJ) transmitter. ACh blockers and acetylcholine receptor (AChR) antibodies reduced responses to neuronally evoked contractions (Fuller, 2006; Mallozzi, 2005; Vacher, 2007). We used a histochemical approach to further confirm the presence of ACh and its receptor at NMJs. Whole mounts and acrylic-embedded sections were treated with antibodies that were fluorescent, enzymatic, or electron-opaque. Polyclonal anti-ACh confirmed the presence of ACh in horseshoe crab leg nerves. Monoclonal anti-AChR demonstrated anti-AChR in some nerves, though less strongly. Immunoelectron microscopy has produced confirmatory micrographs for ACh.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2010

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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