Promensil is an over the counter plant based hormonal supplement used to treat menopausal symptoms, and is considered safer than human estrogen treatments. This project evaluates the model cell line, T47D-KBluc, which synthesizes luciferase in response to estrogen receptor binding, which can be used to quantify the binding affinity of each hormone. Using both luciferase assays and PCNA, the cell was examined for its growth and estrogen receptor activity when exposed to Promensil in order to determine that T47D-KBluc is responsive to estrogen like compounds. The findings can be used to support future studies of Promensil and its components in order to develop a better understanding of phytoestrogen drugs.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Bemis, Randal Glendon
• Sejour, Leinal A.

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042513-101512

Advisor

• Buckholt, Michael Allan
• Rulfs, Jill

Year

• 2013

Date created

• 2013-04-25

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright

Last modified

• 2021-01-29