Faculty Advisor

Buckholt, Michael Allan

Faculty Advisor

Rulfs, Jill

Abstract

Promensil is an over the counter plant based hormonal supplement used to treat menopausal symptoms, and is considered safer than human estrogen treatments. This project evaluates the model cell line, T47D-KBluc, which synthesizes luciferase in response to estrogen receptor binding, which can be used to quantify the binding affinity of each hormone. Using both luciferase assays and PCNA, the cell was examined for its growth and estrogen receptor activity when exposed to Promensil in order to determine that T47D-KBluc is responsive to estrogen like compounds. The findings can be used to support future studies of Promensil and its components in order to develop a better understanding of phytoestrogen drugs.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2013

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

Share

COinS