Faculty Advisor

Adams, David S.

Abstract

Reactive oxygen species (ROS) are reduced forms of oxygen created as byproducts of cellular metabolic processes. ROS cause oxidative damage to many organic molecules within the cell, especially to DNA. OXR2 provides cells with protection against this damage, but its biochemical and functional properties have not yet been studied in depth. The purpose of this project was to create and purify a His-tagged version of human OXR2 and to induce a FLAG-tagged OXR2 in E. coli. These tagged proteins will be used in studies of this protein and its mechanism.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2009

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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