Faculty Advisor

Adams, David S.

Abstract

Recent literature has shown that Matrix and Scaffold Attachment Regions (MARs or SARs) are DNA domains that enhance transcriptional frequency and increase gene expression levels in cells. Research has shown that cell lines containing MARs or SARs are more stable and more productive. Researchers at Abbott Bioresearch Center in Worcester, MA have found two possible MAR regions in an antibody producing cell line. In order to determine whether these regions function as MARs, and if the observed high levels of expression are specific to this cell line, the sequences in question were inserted upstream of the reporter gene, Enhanced Green Fluorescent Protein (EGFP) and tested using FACS analysis. The data indicated the sequences under investigation indeed increased expression of EGFP in stable cell lines, indicating that these sequences can be used to improve transfection and establish high expressing, stable CHO cell lines.

Publisher

Worcester Polytechnic Institute

Date Accepted

March 2005

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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