Recent studies from the Melikian Laboratory demonstrate that KCNK3, a potassium channel found in humans, relies on 14-3-3-Beta for PKC stimulated endocytosis. This MQP examined whether or not the dopamine transporter protein (DAT), a related membrane protein, also relies on 14-3-3-Beta for PKC stimulated endocytosis. Data shows that 14-3-3-Beta co-localizes with DAT at the surface of PC12 cells and suggests that 14-3-3-Beta is needed for DAT PKC induced endocytosis to occur in SK-N-MC cells.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Barton, Steven M.

Contributors

• Stevens, Zack

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042910-141235

Advisor

• Heilman, Destin

Year

• 2010

Sponsor

• University of Massachusetts Medical School

Date created

• 2010-04-29

Resource type

• Major Qualifying Project

Major

• Biochemistry

Rights statement

• In Copyright