Faculty Advisor

Adams, David S.

Abstract

Recently, it has been discovered that null mutations in progranulin is a cause of familial frontotemporal dementia. To understand the role of progranulin in this neurodegenerative disorder, transgenic null mice and in vitro knockdown of mRNA for progranulin were analyzed. After verifying the transgenic mice using Polymerase Chain Reaction to verify progranulin knockdown, the HomeCageScan System and the rotarod test were performed to investigate motor neuron function. In vitro progranulin knockdown in NSC-34 cells was confirmed using RNA extraction and northern blots. The cells that exhibited at least 50% knockdown will be further analyzed in the morphological changes associated with the progranulin haploinsufficiency.

Publisher

Worcester Polytechnic Institute

Date Accepted

August 2008

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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