Faculty Advisor

Buckholt, Michael Allan

Faculty Advisor

Mathews, Lauren M.

Abstract

Amplified fragment length polymorphisms are a PCR-based genetic tool used to mark the presence or absence of alleles in a DNA sample. This tool has typically been used for genetic analysis in plants, but research is now being done to see if it is a reliable source for paternity analysis in animals. Samples of Orconectes limosus were collected from Quinebaug River during a previous study and mated to produce offspring. A blind AFLP analysis was conducted to see if juveniles from known mothers could be matched to their appropriate fathers. The correct father was determined in two of five families.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2013

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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