Faculty Advisor

Adams, David S.

Abstract

RNA interference (RNAi) is a powerful mechanism for gene silencing that holds great promise for therapeutic applications. To design a drug, scientists must find siRNA sequences capable of strong mRNA silencing and effective methods to deliver the siRNA to target tissues. In situ hybridization techniques enable the visualization of the effectiveness of siRNAs. This report demonstrates the visualization of RNAi mediated gene silencing by using a new, highly sensitive bDNA probe-based in situ hybridization assay known as ViewRNA. The ViewRNA System showed in both a qualitative and semi-quantitative manner the reduction of SOD1 mRNA after cells were transfected with siRNA targeting the gene.

Publisher

Worcester Polytechnic Institute

Date Accepted

May 2009

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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