Faculty Advisor
Adams, David S.
Sponsor
RXi Pharmaceuticals
Abstract
RNA interference (RNAi) is a powerful mechanism for gene silencing that holds great promise for therapeutic applications. To design a drug, scientists must find siRNA sequences capable of strong mRNA silencing and effective methods to deliver the siRNA to target tissues. In situ hybridization techniques enable the visualization of the effectiveness of siRNAs. This report demonstrates the visualization of RNAi mediated gene silencing by using a new, highly sensitive bDNA probe-based in situ hybridization assay known as ViewRNA. The ViewRNA System showed in both a qualitative and semi-quantitative manner the reduction of SOD1 mRNA after cells were transfected with siRNA targeting the gene.
Publisher
Worcester Polytechnic Institute
Date Accepted
May 2009
Major
Biology and Biotechnology
Project Type
Major Qualifying Project
Copyright Statement
All authors have granted to WPI a nonexclusive royalty-free license to distribute copies of the work, subject to other agreements. Copyright is held by the author or authors, with all rights reserved, unless otherwise noted.
Accessibility
Unrestricted
Advisor Department
Biology and Biotechnology