Faculty Advisor

Adams, David S.

Center

University of Massachusetts Medical Center

Abstract

SERCA3-ATPases help regulate intracellular Ca2+ levels, critical for maintaining normal insulin secretion. To investigate the potential role of the SERCA3 pathway in diabetes, we assayed the cellular levels of SERCA3 and insulin proteins in EndoC-beta-H1 human pancreatic cell lines derived from normal and type-2 diabetic patients (T2D) using immunofluorescence, immunohistochemical microscopy, and western blots. The data indicate that both of the proteins are significantly lower in T2D patients. To determine whether SERCA3 is required for insulin secretion, we knocked down the ATP2A3 gene of the SERCA3 pathway in EndoC- beta-H1 cells, and verified the knockdown by qRT-PCR and Western blots. In the future, the knockdown cells will be assayed for insulin levels.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2016

Major

Biology and Biotechnology

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

Project Center

University of Massachusetts Medical Center

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