Roche Molecular Systems, Inc.
An aptamer is an oligonucleotide that specifically and reversibly binds an enzyme, influencing its activity. DNA polymerase enzyme synthesizes DNA. The benefits of adding aptamer to DNA polymerase include: Hot Start PCR compatibility, polymerase stability when in complex with aptamer, and protection against harmful environmental conditions. However, the underlying mechanisms that account for these properties are not well understood. Through the development of a proteinase K challenge experiment, this project determined the location of aptamer binding on DNA polymerase and its functional implications.
Worcester Polytechnic Institute
Biology and Biotechnology
Major Qualifying Project
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Chemistry and Biochemistry