Faculty Advisor

Heilman, Destin

Abstract

An aptamer is an oligonucleotide that specifically and reversibly binds an enzyme, influencing its activity. DNA polymerase enzyme synthesizes DNA. The benefits of adding aptamer to DNA polymerase include: Hot Start PCR compatibility, polymerase stability when in complex with aptamer, and protection against harmful environmental conditions. However, the underlying mechanisms that account for these properties are not well understood. Through the development of a proteinase K challenge experiment, this project determined the location of aptamer binding on DNA polymerase and its functional implications.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2014

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Chemistry and Biochemistry

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