Faculty Advisor

Pins, George

Faculty Advisor

Rolle, Marsha W.

Abstract

Several co-culture systems involving fibroblasts and endothelial cells have been used to characterize angiogenesis. However, these models have not been translated into therapies for chronic non-healing wounds. We developed an approach that may facilitate in vivo neovascularization and serve as an in vitro tool to study angiogenic mechanisms. Self-assembled collagen microthreads populated with human dermal fibroblasts (HDFs) were extruded using a novel method prior to surface seeding of human umbilical vein endothelial cells (HUVECs). Fluorescence microscopy confirmed the presence of both cell types after 48 hours of co-culture. These results demonstrate that a viable co-culture system can be established on collagen microthreads with these seeding methods.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2009

Major

Biomedical Engineering

Major

Chemical Engineering

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biomedical Engineering

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