Faculty Advisor

Pins, George


Skin provides the body's barrier function and therefore must heal quickly when wounded. Non-healing wounds require grafting or application of bioengineered skin substitutes(BES), which lack the ability to rapidly regenerate. In order to enhance the rate of regeneration of BES, there is a need for the development of an in vitro wounding model for BES, which is the goal of this project. A device was manufactured that created full-thickness, reproducible wounds to foreskin tissue, a model tissue for BES. Histology showed that a 90 second cryoburn from a liquid-nitrogen-cooled stainless-steel rod followed by a 72-hour culture period was successful in producing the desired wound. The result of this project is a model system and device that will be useful in the study of in vitro BES healing.


Worcester Polytechnic Institute

Date Accepted

April 2010


Biomedical Engineering

Project Type

Major Qualifying Project



Advisor Department

Biomedical Engineering