Page, Raymond L.
Spinal cord injuries in canines can lead to loss of neurological function. Exosomes from the ECM are a new method being explored for therapeutic applications. This project aimed to design and test an exosome delivery vehicle that could release exosomes for spinal cord therapy. The exosomes were concentrated by tangential flow filtration and ultracentrifugation. They were loaded into fibrin and methacrylated chondroitin sulfate. Material degradation and exosome release were tested by nanoparticle tracking analysis (NTA) and gel electrophoresis. Fibrin degraded faster and more particles were released. High molecular weight proteins were present after material degradation and released into fluid. This project shows exosomes can be loaded into a device and released for therapeutic purposes.
Worcester Polytechnic Institute
Major Qualifying Project