Stem cell-based therapy shows promise as a strategy to treat a number of neurodegenerative diseases and injuries, such as Alzheimer’s Disease and Spinal Cord Injury. Currently, only 5-10% of neural stem cells (NSCs) survive the transplantation due to the procedure and the hostile environment once implanted. Therefore, a high-throughput method for encapsulating the NSCs, as well as producing uniform microgel spheres is necessary. A microfluidic system was designed to encapsulate live cells in microgel spheres. Specifically, a flow-focusing microfluidic device was used for its ability to produce a more consistent microgel sphere size as compared to other devices. A protocol for cell encapsulation was developed in order to successfully encapsulate live cells in microgel spheres.
Worcester Polytechnic Institute
Major Qualifying Project
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