Wobbe, Kristin K
An important immune response to pathogens in A. thaliana and other plants is the hypersensitive response (HR). Turnip Crinkle Virus (TCV) is unique in the Carmovirus genus for its ability to suppress the HR in systemic infection of A. thaliana. It is hypothesized that the p8 viral movement protein of TCV is responsible for this ability. It should be possible to test if p8 suppresses the HR by replacing it with a homologous gene. Genetic modifications were made to an expression vector containing a dsDNA copy of the TCV ssRNA genome, pT1D1131180L. The inserted homologous gene was p7, from the related Carnation Mottle Virus.
Worcester Polytechnic Institute
Major Qualifying Project
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