Faculty Advisor

Adams, David S.

Abstract

Studies have shown that an innate immune response occurs during viral infection of mammalian cells when two protein components, TLR-3 and TRIF, bind together after recognition of foreign RNA inside a cell. The TLR-3/TRIF interaction was used to design a fluorescence-based two-hybrid system for rapidly detecting Flaviviral infections. A plasmid encoding TRIF was engineered to express half of a Venus (YFP) reporter, while TA cloning was used to confirm the clonability of the TLR-3 gene. Future experiments include cloning the TLR-3 gene into the Venus vector, and transfecting the fusion proteins into Vero cells. Upon addition of a sample containing viral RNA to this cell-line, the modified TLR-3 and TRIF proteins should dimerize, allowing the YFP to assemble, causing the cell to fluoresce.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2010

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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