Studies have shown the cancer-cell killing abilities of proteins expressed by the third open reading frame of members of the Circoviridae virus family. PCV1 was discovered to possess a VP3 homologous to those of other circoviruses, suggesting that it too may have apoptotic activity. Previous studies have shown that cellular localization may be responsible for the killing action of the other VP3 proteins. In order to better study the activity of PCV1 VP3, truncation mutants have been developed. Furthermore, the apoptotic abilities of wild type vector constructs were assessed for the first time. The assay illustrates that apoptosis occurs by a capases-3-7 pathway; leading us to question the impact of localization, and structural differences of PCV1 may be responsible for functionality.
Worcester Polytechnic Institute
Major Qualifying Project
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Chemistry and Biochemistry