Mapping of HTLV-1 Tax Functional Domain Conferring Cellular Senescence
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open in viewerTax is a human T-lymphotrophic virus type 1 (HTLV-1) protein that predisposes transformation of certain cell types by inducing cellular senescence. To map the Tax domain that confers this senescence, PCR-based truncation mutagenesis was used. PCR products included full length tax and mutants lacking the C-terminal quarter, C-terminal half, and N-terminal quarter of the protein. These mutants and the wild type were cloned into a yeast plasmid vector under the control of a GAL1 promoter and used to transform Y187 S. cerevisiae. After Trp- selection and galactose induction, the transformed yeast phenotypes were observed. Based on comparison of wild type and truncation phenotypes, it was concluded that the senescence-conferring domain could be located in the C-terminal region of the protein.
- This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
- Creator
- Publisher
- Identifier
- E-project-042508-125134
- Award
- Advisor
- Year
- 2008
- Date created
- 2008-04-25
- Resource type
- Major
- Rights statement
- Last modified
- 2023-01-20
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