Faculty Advisor

Heilman, Destin

Faculty Advisor

Wobbe, Kristin K

Abstract

Tax is a human T-lymphotrophic virus type 1 (HTLV-1) protein that predisposes transformation of certain cell types by inducing cellular senescence. To map the Tax domain that confers this senescence, PCR-based truncation mutagenesis was used. PCR products included full length tax and mutants lacking the C-terminal quarter, C-terminal half, and N-terminal quarter of the protein. These mutants and the wild type were cloned into a yeast plasmid vector under the control of a GAL1 promoter and used to transform Y187 S. cerevisiae. After Trp- selection and galactose induction, the transformed yeast phenotypes were observed. Based on comparison of wild type and truncation phenotypes, it was concluded that the senescence-conferring domain could be located in the C-terminal region of the protein.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2008

Major

Biology and Biotechnology

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Chemistry and Biochemistry

Advisor Department

BC

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