Faculty Advisor

Buckholt, Michael Allan


TB is a disease caused by M. tuberculosis; it’s one of the leading causes of death globally from a single pathogen. Antibiotic resistant TB has developed. This study worked to discover new antibiotics and study how they affect TB. A WPI class collects soil microbes to find new antibiotics. Our project worked with microbes that were able to inhibit the growth of M. smegmatis. Four isolates showed potential antibiotic activity; extracts from these were tested to discover the responsible compound. One extract shows promise of having an antibiotic compound. To understand antibiotic effects on gene expression, data from RNAseq analysis of oxidative stress on the M. smegmatis genome was done. Hypothetical and DNA repair proteins were identified as the most affected.


Worcester Polytechnic Institute

Date Accepted

April 2018


Biology and Biotechnology

Project Type

Major Qualifying Project



Advisor Department

Biology and Biotechnology