Wobbe, Kristin K.
University of Massachusetts Medical Center
Dr. Paul Clapham
The ability of HIV-1 to interact with receptors on the host cell plays a major role in determining its tropism. The T283N mutation in the CD4bs of HIV has been shown to drastically affect macrophage tropism. More recently an E153G mutation located in the V1 loop which modulates the V3 loop to prime low CD4 use has also been shown to confer high levels of macrophage infectivity. This project explores whether these residues involved in the non-macrophage-tropic phenotype of transmitted R5 viruses or effect sensitivity to entry inhibitors, soluble CD4, b12 and Maraviroc. The results presented confirm that substitutions at 153 and 283 affect macrophage-tropism of some viruses and/or shift sensitivity to entry inhibitors.
Worcester Polytechnic Institute
Major Qualifying Project
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Chemistry and Biochemistry