Student Work

Characterizing the DNA-Binding Site Specificities of Cis2His2 Zinc Fingers

Public

Downloadable Content

open in viewer

The ability to modularly assemble Zinc Finger Proteins (ZFPs) as well as the wide variety of DNA sequences they can recognize, make ZFPs an ideal framework to design novel DNA-binding proteins. However, due to the complexity of the interactions between residues in the ZF recognition helix and the DNA-binding site there is currently no comprehensive recognition code that would allow for the accurate prediction of the DNA ZFP binding motifs or the design of novel ZFPs for a desired target site. Through the analysis of the DNA-binding site specificities of 98 ZFP clones, determined through a bacterial one-hybrid selection system, a predictive model was created that can accurately predict the binding site motifs of novel ZFPs.

  • This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
Creator
Publisher
Identifier
  • E-project-042512-185614
Advisor
Year
  • 2012
Sponsor
Date created
  • 2012-04-25
Resource type
Major
Rights statement

Relations

In Collection:

Items

Items

Permanent link to this page: https://digital.wpi.edu/show/8c97ks10v