Adams, David S.
University of Massachusetts Medical School
The activation of signaling by erythropoietin (Epo) is a critical part of the differentiation and maturation of blood cell progenitors. Stat5, a transcription factor that is phosphorylated indirectly by the binding of Epo to the erythropoietin receptor (EpoR), has been shown to be constitutively active in leukemia and other myelo-proliferative disorders. Using PCR amplification and vector cloning, a fusion protein of emerald GFP and SOCS3 (a negative regulator of Stat5) was created to study the time course of activation of Stat5 activation in erythroid progenitors in both normal and abnormal erythropoiesis. The results of further studies using this fusion protein may eventually lead to improved or novel treatment options for patients with leukemia.
Worcester Polytechnic Institute
Major Qualifying Project
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Biology and Biotechnology