Faculty Advisor

Billiar, Kristen

Abstract

The aim of this project was to design and validate a device to cyclically stretch human cells on tunable stiffness substrates to enable the study of these two mechanical stimuli simultaneously. FEA indicated that squat stretching should yield near pure uniaxial behavior. Polyacrylamide (PA) hydrogel was utilized as the compliant substrate for tissue culture in a temperature controlled, sterile environment. Testing verified that PA gels have sufficient durability in squat configurations and can reach 20% strain at rates up to 10%/sec. This device represents the first method available for mechanobiological studies into the response to combined stretch and stiffness stimuli.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2009

Major

Mechanical Engineering

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biomedical Engineering

Share

COinS