Faculty Advisor

Adams, David S.

Center

University of Massachusetts Medical Center

Abstract

The Aroian lab previously identified a list of differentially regulated proteins when Caenorhabditis elegans was exposed to Cry5B. The actin-interacting proteins showed the most notable changes. Using genetic interacting assays with known pathways, hypersensitivity assays, pore repair assays, and endocytosis assays, we investigated the potential role of four actin-binding genes: dbn-1, erm1, plst-1, and F38E9.5. Knockdown of dbn-1, erm1, and plst-1 showed increased susceptibility to toxin Cry5B exposure than empty vector, suggesting these genes play a functional role in toxin defense.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2016

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

Advisor Program

Biology and Biotechnology

Project Center

University of Massachusetts Medical Center

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