The Aroian lab previously identified a list of differentially regulated proteins when Caenorhabditis elegans was exposed to Cry5B. The actin-interacting proteins showed the most notable changes. Using genetic interacting assays with known pathways, hypersensitivity assays, pore repair assays, and endocytosis assays, we investigated the potential role of four actin-binding genes: dbn-1, erm1, plst-1, and F38E9.5. Knockdown of dbn-1, erm1, and plst-1 showed increased susceptibility to toxin Cry5B exposure than empty vector, suggesting these genes play a functional role in toxin defense.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Zamaitis, Tammy Kate

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042716-175040

Advisor

• Adams, David S.

Year

• 2016

Sponsor

• University of Massachusetts Medical Center
• Raffi Aroian

Date created

• 2016-04-27

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright