Faculty Advisor

Mathews, Lauren M

Abstract

The Blackstone Valley Watershed provides a unique opportunity to study phylogeographic relationships between two related crayfish species, Orconectes virilis and O. quinebaugensis. Crayfish were collected from various sites in the watershed and individuals were analyzed for the COI mitochondrial gene, a microsatellite allele (Ov54), and pleopod and chelae morphology. The COI gene had two haplotypes associated with O. virilis and with O. quinebaugensis. The Ov54 locus yielded three genotypes, AA, BB, and AB. The Ov54 genotype was dependent on COI haplotype (p<0.05) and both Ov54 genotype and COI haplotype were significantly related to pleopod angle. We consider these results in the context of possible introgression between the two species.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2009

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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