Faculty Advisor

Adams, David S.

Abstract

The dopamine (DA) transporter (DAT) is a sodium symporter (NSS) transporter protein that removes dopamine from the synaptic cleft to the pre-synaptic nerve terminal. DAT is regulated by signal and protein cascade pathways, and thus is trafficked to and from the nerve terminal cell surface to modulate DA uptake. This project investigates the role of calmodulin (CaM) in the signaling pathway affecting DAT trafficking, and focuses on whether CaM is required for PKC-mediated DAT surface expression. DA transport assays using a CaM inhibitor, CGS 9343B, revealed a trend toward CaM’s ability to increase DAT function. Preliminary results suggest that CaM is able to increase DAT function, but whether it affects DAT surface levels is the foundation for further investigations.

Publisher

Worcester Polytechnic Institute

Date Accepted

January 2014

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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