Adams, David S.
UMass Medical School
The Dopamine Transporter (DAT) is a trans-membrane protein that is part of the SLC6 gene family. Protein Kinase C (PKC) has been shown to acutely down regulate DAT via membrane trafficking away from the plasma membrane. The present study was performed to determine the role of PKCBeta, a subtype of PKC, in DAT trafficking. Dopamine uptake assays and cell surface protein biotinylations were performed to access the effect of PKCBeta's inhibition. Wild Type DAT function and surface expression were not sensitive to PKCBeta inhibition. In contrast, mutating DAT residues 587-589 (DAT 3A) revealed striking PKCBeta sensitivity, resulting in significantly increased DAT function and surface expression following 20-minute treatment with the PKCBeta inhibitor LY 379196.
Worcester Polytechnic Institute
Major Qualifying Project
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Biology and Biotechnology