Faculty Advisor

Buckholt, Michael Allan

Faculty Advisor

Farny, Natalie

Abstract

Annually, 2.5 billion individuals risk contracting the dengue virus and a lack access to a simple diagnostic test for this disease. We aimed to design a diagnostic test for dengue infection by genetically engineering E. coli to express surface dengue envelope proteins. The resulting bacteria should agglutinate with dengue antibodies to form bacterial films, indicating an immune response to dengue virus in a patient serum sample. Here we demonstrate a successful proof-of-concept using E. coli expressing GFP on the surface, and are able to detect anti-GFP antibodies to a sensitivity of 0.005 ug/mL. Our system lays the groundwork for the development of a cost effective dengue screening tool, which will enable diagnosis on a broad scale and improve survival.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2017

Major

Biology and Biotechnology

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

Advisor Department

BIO

Share

COinS