Faculty Advisor

Dominko, Tanja

Abstract

Antimalarial artemisinin-derived compounds have attenuated fibrosis in preclinical models, but the mechanisms by which this inhibition occurs are not well-understood. We investigated the effects of artesunate on the emergence of the myofibroblast, which causes fibrosis. Treatment of human dermal fibroblasts with artesunate antagonized fibroblast activation and ECM deposition. Artesunate-treated fibroblasts demonstrated decreased proliferation and increased apoptosis. qRT-PCR analysis showed artesunate-mediated downregulation of profibrotic genes, and upregulated expression of growth inhibitors and MMPs. These data suggest that the antifibrotic mechanisms of artesunate may be mediated by antagonistic effects towards profibrotic gene expression, and by induction of myofibroblast apoptosis.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2018

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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