Faculty Advisor

Adams, David S.

Abstract

ARHI is a tumor suppressor gene inactive in up to 60% of breast, pancreatic and ovarian cancers. To test its role in sebaceous tumors, immunofluorescence, Western blots, and PCR were used to assay ARHI mRNA and protein presence in normal human sebaceous cells, and its absence in human sebaceous tumor cells. To test the role of Lef/TCF transcription factors on ARHI expression, the levels of ARHI mRNA in normal sebaceous, telogen stem cells, and tumor cells were compared with ARHI levels in a LEF/TCF knockdown line. The data indicated lower ARHI expression in the LEF/TCF knockdown cells, evidence of a possible relationship. The data support the role of ARHI in sebaceous tumor formation in tissues, but indicate no significant difference in ARHI mRNA or protein levels in cultured cells.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2008

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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