The goal of this project was to develop high-throughput protein and peptide loading of beta glucan particles (GPs) for vaccine development in 96-well plates. After establishing ideal loading conditions to be similar to those of standard GP loading, peptide binding using N-succinimidyl S-acetylthioacetate (SATA) and a new binding method using 2,2'-dithiolethyl bis(4-azido-2,3,5,6-tetrafluorobenzoate) (DAT) showed that the DAT GPs bound peptide more efficiently than the SATA GPs. GP-mediated peptide delivery to OT-II T-cells showed that DAT-derivatized GPs stimulated a stronger immune response than linear PEI/SATA-derivatized GPs. Immunological testing for Ova protein-loaded GPs versus IgG- and lysozyme-loaded GPs showed that both Ova protein- and IgG-loaded GPs stimulated a T-cell response.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Jones, Lindsay S.
• Duca, Zachary A.

Contributors

• Ostroff, Gary

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-050114-163708

Advisor

• Politz, Samuel M.
• Heilman, Destin

Year

• 2014

Sponsor

• University of Massachusetts Medical School

Date created

• 2014-05-01

Resource type

• Major Qualifying Project

Major

• Interdisciplinary
• Biology & Biotechnology

Rights statement

• In Copyright